FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49983933
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG122300.012.43476245
ACCCGGC97000.012.2767115
GGCATAG128550.011.93495635
ATAGCTC94600.011.58089239
ACGGACA105050.010.81666518
CATAGGG141200.010.78633237
GAGCTTA146000.010.70899544
TCAGACG91600.010.4436632
ATAGGGG148050.010.43902438
TAGCTCT106150.010.34203940
GTCACTC192900.010.3130128
GCATAGG149000.010.19151236
CGGACAC111300.010.10915113
TCACCCG124900.010.0742193
CCGGACA120350.010.00390812
GGTCACT197050.09.91367
CGGACAG112950.09.84083219
TGGCATA164100.09.67763934
CACCCGG127100.09.6166954
TGATAGC119850.09.59185737
Produced by FastQC (version 0.11.5)