FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45260592
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT703260.15538020359963475No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG506180.1118368049626925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC141300.024.1896464
GACCGCG144800.023.6825715
TTCGACG23250.023.4143456
ACCGCGT146350.023.409756
CAGACCG156750.022.135573
AACGCTA225250.021.18454723
ACGCTAC227000.021.04172924
CGCTACC233650.020.3977725
AAAACGC246750.019.37350321
TCAGACC185350.019.096292
GAAAACG252800.018.9261720
TCGACGA30750.018.7272247
CCGCGTT183550.018.6035567
CGGTATA39200.018.5922220
AAACGCT262800.018.2592122
TCACGCT272950.018.0978628
TTCACGC290450.017.1546137
CGTGGTT286650.016.84813941
GTTTTCG256400.016.82696545
ACGCTCA301200.016.81842210
Produced by FastQC (version 0.11.5)