FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45260592
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG515170.11382308035210853No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN482250.10654964477707228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG156150.016.38074345
ATAGGGG168150.015.3439438
GTTGTCG32550.015.20501326
GGCATAG168700.015.18800235
CATAGGG184150.014.03484437
GAGCTTA186250.014.01137244
TGGCATA195250.013.30711634
ATATAGC43150.013.2970236
TGTCGTT37900.013.23662928
GGAGGTC259450.013.2344834
GTCACTC259400.013.0156568
GGGAGGT279250.012.9708663
ACTCTCC263450.012.81599411
GCATAGG203400.012.70727436
GGTCACT262350.012.6532837
TATAGCT46950.012.1756017
AGGGATC140800.011.8075632
TAGCGTA8400.011.78237137
GCTAAGT43000.011.411201514
TAGGGGA295900.011.20528339
Produced by FastQC (version 0.11.5)