FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50208967
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1023770.2039018249469263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG50550.029.4207826
TCGACGA60000.025.0495037
CGACGAG58100.024.977948
GTTCGAC60300.024.7757765
CGGTATA81600.023.98844520
GCCGGCT289150.019.9807991
CCGGCTT325250.019.729382
TCACGCT324650.019.3842988
ACCGCGT103650.019.254326
GACCGCG104200.018.9584715
AGTTCGA81650.018.8762074
TTCACGC336600.018.8365257
AGACCGC110650.018.402534
ATGACGG103100.018.33131616
CAGTTCG86950.018.269033
ACGCTAC337000.018.1464924
AACGCTA338300.018.07675623
GACGGTA111450.017.72505218
ACGCTCA359450.017.71417610
CGCTACC346800.017.58179525
Produced by FastQC (version 0.11.5)