FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46862159
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT504170.10758573884741417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT134600.026.67896545
AGACCGC156050.023.1418674
ACCGCGT157000.022.9875056
AGAACGA164750.022.05553244
GACCGCG167050.021.618015
CAGACCG169650.021.4999353
CCGCGTT168450.021.4242957
AATACGG176650.020.44403632
CGCGTTC176550.020.4159418
AAGAACG187200.019.3023543
ATACGGA124850.019.28336533
CAATACG192000.018.84507831
TACGGAG128600.018.82603834
TCAGACC201200.018.655472
ATACGGG65100.018.6292533
GCGTTCT209600.017.3464039
TACGGGG73800.016.9209334
CCCAATA218200.016.71634729
CCAATAC235350.015.70856330
CTCAGAC281800.015.3245711
Produced by FastQC (version 0.11.5)