FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46862159
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24000.014.43549826
AGCTTAG152950.014.399445
GGCATAG161800.013.61311535
ATAGGGG170000.013.1142638
GGGAGGT278550.012.2612283
GAGCTTA183850.012.11346444
TGGCATA187900.011.77010934
CATAGGG189450.011.74477937
GGAGGTC287400.011.2317914
GCATAGG200350.011.08347836
TGTCGTT30650.010.935348528
GGTCACT286250.010.8873937
ACTCTCC294800.010.73144111
ATATAGC39200.010.3894766
GTCACTC310200.010.3152728
ATAGCTC69400.010.11409839
TAGGGGA308900.09.90496639
GCTAAGT36250.09.74441214
ACCCGGC79300.09.736485
TCACCCG84500.09.6183823
Produced by FastQC (version 0.11.5)