FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53583405
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC60950.023.51720632
TCGTATG65000.022.53254545
CGTCCCG79300.019.49186135
GTCCCGA83900.017.64433736
GTCCGTT40850.017.544471
TGCGCGT37450.017.24140745
ACGGTAC148500.017.1044945
CGTCTGA90500.016.35733816
CCCGTAT35750.016.3278471
ACGCTAC143400.016.19267524
AACGCTA143450.016.1556323
TACGGTA158150.016.10352944
ACGTTCG23050.015.4209726
TTACGGT168550.015.17689143
CCTTACG168500.015.16814341
CGCTACC154600.014.9765925
ACACGTC98550.014.90702713
CTCGTAT96950.014.89810644
ACGTCTG100550.014.63291915
ATGCGCG45050.014.38273644
Produced by FastQC (version 0.11.5)