FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50347187
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1622540.3222702392489177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT317700.027.0347041
TCACGCT376950.024.5500478
CCGGCTT393100.023.8165052
AACGCTA383400.023.6147423
ACGCTAC385550.023.47138624
TTCACGC412000.022.8547047
CGCTACC395650.022.81534825
AAAACGC404600.022.44412221
ACGCTCA426250.021.95340310
GAAAACG413600.021.92853420
AGACCGC92450.021.6116394
CACGCTC432750.021.4572859
AAACGCT423700.021.44298422
CGGCTTC456700.021.3421963
GCTACCT477900.019.13819126
CAGACCG106450.019.1286623
CGTGGTT479450.018.84655841
CTACCTC486900.018.79369527
GTTTTCG483950.018.49462545
TCGTGGT496800.018.15208440
Produced by FastQC (version 0.11.5)