FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45493983
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT692580.15223551650775444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT184900.024.4309026
AGACCGC187100.024.291394
GACCGCG193650.023.3651925
AACGCTA130800.023.0994723
CCGCGTT199400.022.6768997
GAACGAT199850.022.5807145
ACGCTAC133950.022.55851424
CAGACCG204400.022.400533
CGCGTTC203800.022.1761958
CGCTACC138750.021.68095825
AATACGG223150.020.22170832
AAAACGC152500.020.03293221
AGAACGA225950.020.02214244
ATACGGA171950.019.42248733
TCAGACC238900.019.415342
AAACGCT160200.019.0842222
GAAAACG162650.018.90728220
TACGGAG177200.018.83424234
GCGTTCT242250.018.7120829
CAATACG242850.018.6647331
Produced by FastQC (version 0.11.5)