FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48275490
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC71050.012.0393155
TCAGACG110650.011.5778942
GTTGTCG25700.010.85466726
TCACCCG85150.010.1532683
CAGACGT129850.09.9524323
CCGGACA90900.09.9257812
CGTCGAT21850.09.78264612
CGGACAC90750.09.76894713
CACCCGG88250.09.6419774
CGGACAG86200.09.552340519
ATAGCTC82550.09.53855339
GCGTCGA23800.09.3596111
AGACGTG140050.09.2100194
CGGCCCG105700.08.8134878
TGCGTCG26450.08.76211210
AGGGATC145550.08.5697142
GGCATAG82750.08.56427835
TAGCTCT93100.08.5539440
ATAGGGG90100.08.18971638
ACGGACA103900.08.18487918
Produced by FastQC (version 0.11.5)