FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46271997
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC560800.12119641173040359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT39100.018.0260681
CTCGGTT100100.017.434161
TTCGACG18800.017.111746
AGACGCT259450.016.18853641
ATGCGCG25800.016.0442844
CTCCGTT68250.015.7379851
CCCGTAT32300.015.5466131
TCGGTTT114350.015.3845822
ACGTTCG21400.015.1378696
CCCCGCA263950.015.12102735
AAGCGGC264150.015.09100728
GCTCGGC236100.015.02664645
CCGCAGA286800.015.00561137
CGGTTTT104600.014.8829663
GTCGGTT44500.014.826581
GTCCGAT24650.014.7076191
GCACTAC65800.014.70589325
TGCGCGT29950.014.64752145
ACGCTAC109550.014.29600624
AACGCTA109750.014.2688623
Produced by FastQC (version 0.11.5)