FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46271997
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG520630.11251513523395154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG168000.015.01062345
GGCATAG176500.014.198729535
ATAGGGG191650.013.27573338
GAGCTTA195950.012.94989444
GTCACTC259500.012.5909478
GGGAGGT287500.012.5070783
GGTCACT258900.012.4984447
TGGCATA204650.012.40031134
CATAGGG208450.012.195065537
ACCCGGC91300.012.1509295
GAGGTCA281800.012.0578355
ACTCTCC282250.011.91917611
GGAGGTC286650.011.8538214
GCATAGG216650.011.78543636
AGGTCAC288050.011.7492216
ACGGACA93450.011.48587118
CGGACAC95950.011.09319313
TCACCCG111500.010.8371813
TCACTCT317750.010.6227219
CGCCGTA19500.010.49806345
Produced by FastQC (version 0.11.5)