FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45295694
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA7900.012.81358737
AGCTTAG99300.012.3007545
ACCCGGC77950.012.0969945
GGCATAG98500.012.08113335
CATAGGG104700.011.36565137
ATAGGGG108700.011.21639138
ATAGCTC84800.010.87603139
GCATAGG111400.010.78305536
ATAGCGT9200.010.75847336
CGGACAC91050.010.62855613
ACGGACA90300.010.61592318
GAGCTTA118000.010.35139444
TCAGACG78100.010.344342
TCACCCG99050.010.1330743
CCGGACA99350.09.89922612
GTCACTC160900.09.6092578
CGCGTTC18850.09.5511114
CACCCGG99850.09.5336434
CGGACAG99300.09.51758619
TGGCATA127000.09.40559334
Produced by FastQC (version 0.11.5)