FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48169433
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG837730.1739131951169116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG237100.017.56474345
GTTGTCG30900.017.02641526
GGCATAG251900.016.36113235
ATAGGGG258700.016.1281738
GGGAGGT389850.015.4151613
GAGCTTA273000.015.35433744
TGGCATA271050.015.3234134
GGAGGTC395350.014.80324654
CATAGGG302200.013.75435537
CACCCGG120050.013.5572681
TGTCGTT39700.013.538465528
ACTCTCC430950.013.36609311
GCATAGG320400.013.03004836
TGGGAGG336450.013.024312
GGTCACT446250.012.8930037
GTCACTC456400.012.7636018
ATATAGC49300.012.5673876
GAGGTCA486600.012.2346485
TAGGGGA484150.011.907948539
TATAGCT51400.011.8355897
Produced by FastQC (version 0.11.5)