FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53408363
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1014850.19001705781545858No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG616300.1153939131218083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG39400.025.7555486
CGACGAG45500.021.6098428
TCGACGA49450.020.7941117
GACCGCG257750.020.4360665
ACCGCGT258900.020.4320096
AGACCGC262100.020.1913284
GTTCGAC50700.019.7934475
CCGCGTT271250.019.5100357
CGGTATA64950.019.46883420
CAGACCG276600.019.303683
CGCGTTC276550.019.1845518
GAACGAT283450.018.16957545
TCAGACC321450.016.9055922
GCGTTCT319250.016.6394489
CTCAGAC378200.016.030921
GCCGGCT224550.015.9555721
CCCGTAT35250.015.8334281
CCGGCTT264300.015.7592652
ATGACGG76400.015.66728216
GTCCGTT42050.015.360251
Produced by FastQC (version 0.11.5)