FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48485882
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1968880.40607284404973804No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG588980.12147453561843012No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA501510.10343423267003785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT319200.030.00020445
AGAACGA384900.025.06027844
ACCGCGT416750.023.2151246
AGACCGC416950.023.1609614
CAGACCG428350.022.5865143
CCGCGTT430550.022.4919037
CGCGTTC436800.022.2009328
GACCGCG437450.022.075585
AATACGG451750.021.28721432
ATACGGA454250.021.15027633
TACGGAG454700.021.11452534
AAGAACG460550.020.96484243
TCAGACC464700.020.9454232
GCGTTCT477900.020.3668299
CAATACG474550.020.31652831
ACGGAGA480350.020.01509935
TTCGGCG81100.019.7210944
CTCAGAC536150.019.4069021
TCGGCGC83600.019.02386545
CGGAGAG505800.018.98580736
Produced by FastQC (version 0.11.5)