FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48485882
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3714570.7661137318281639No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA584280.1205051812814295No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG540200.11141387507398545No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG518190.10687440933837193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG155450.018.0101345
GTTGTCG27300.017.34958526
GGCATAG170750.016.18139635
ATAGGGG190400.014.79313738
GAGCTTA192400.014.65562844
TGGCATA192800.014.49359234
CATAGGG195200.014.40694437
ACCCGGC84100.014.0146515
GCATAGG207150.013.5218136
GGGAGGT333900.012.7549373
GGAGGTC312900.012.60621554
ACGGACA93750.012.59456818
TCACCCG99150.012.5634843
ATAGCTC89900.012.55684439
ACTCTCC313200.012.54921211
TGTCGTT38850.012.26223728
GGTCACT318700.012.0671117
GTCACTC335700.011.7633798
GTCGTTC41750.011.73620629
GCTAAGT43250.011.62628914
Produced by FastQC (version 0.11.5)