FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51622080
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG21400.016.50311544
GCGTCGT29250.012.84959611
TCGCCGT29150.012.19266445
GGTCGCC30200.011.69426143
AGCGTCG31250.011.37903810
CGTCGTG34200.010.52913712
CTTGACG151050.010.12670742
TGGTCGC35650.09.78030242
GTCGTGT38200.09.54406313
TTGACGT163650.09.44323543
TCGTGTA38950.09.41803814
GCACCGA86750.09.1275344
CGTGGCG52650.09.0194317
CGACCCG55950.08.52775412
TCTCGGT43650.08.50314836
TCAGACG63900.08.4522972
ATCTCGG43750.08.43232235
CACCGAA96650.08.3322345
GCGGGCT48850.08.15131930
CGCGGTA17750.07.98805511
Produced by FastQC (version 0.11.5)