FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51175682
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT784760.15334627098863088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG32550.024.6077546
GACCGCG173150.022.9088215
ACCGCGT173250.022.8826146
AGACCGC176450.022.5954484
CAGACCG183800.021.8632563
CCGCGTT185250.021.3881937
CGCGTTC192300.020.6625738
TCGACGA41750.019.5624457
CGGTATA51600.019.31643520
CGACGAG40200.019.2533078
GAACGAT211500.018.52132245
TCAGACC238000.017.262532
GCGTTCT239650.016.6363969
ATACGGA236550.016.5791533
AATACGG238850.016.52288232
TACGGAG236150.016.3976834
GTTCGAC49800.016.1291645
CTCAGAC297750.015.7160471
AGAACGA253100.015.69936744
CAATACG263350.015.19077431
Produced by FastQC (version 0.11.5)