FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51175682
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG166400.014.19642738
GTTGTCG34000.014.1601626
AGCTTAG166000.013.93218345
GGCATAG164150.013.89750235
GTCACTC230250.012.7431458
CATAGGG180800.012.67998837
GGGAGGT274200.012.216563
GGTCACT242450.012.2046697
GAGCTTA192350.012.09379344
AGGTCAC256150.012.0177256
GCATAGG192600.011.98479936
ACTCTCC253450.011.83326811
GGAGGTC261350.011.7919124
TGGCATA201900.011.53292534
TGTCGTT43700.011.27454428
GAGGTCA290050.010.9341375
ACCCGGC71700.010.6734835
TATAGCT56250.010.5609717
TAGGGGA286850.010.2666239
GTCGTTC46400.010.23059229
Produced by FastQC (version 0.11.5)