FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51678829
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG22300.019.2709666
GAACGAT127600.018.40884245
TTCGGCG96300.015.98109344
AGACCGC152950.015.8725914
GTCCGTT44200.015.8446481
ACCGCGT154000.015.66206556
TCGGCGC96650.015.45762945
GACCGCG160200.015.0559185
GTTAGTT125750.014.77912444
AACGCTA96350.014.71161823
AGAACGA162050.014.68971344
CCGCGTT164000.014.679517
CGCGTTC164500.014.63489158
CAGACCG169150.014.5918523
ACGCTAC98200.014.4115824
CGGTATA41050.014.19574620
TCGACGA32600.013.9413677
CGCTACC104950.013.50617125
CCCGTAT32200.013.3574011
TACGGAG182600.013.1596234
Produced by FastQC (version 0.11.5)