FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41468934
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG442680.10674979009588237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA19150.017.50341845
AGCTTAG138950.015.44525945
GGCATAG153650.013.93967835
GTTGTCG24450.013.52765326
ACCCGGC95550.013.2814815
TCACCCG99700.013.1801073
GAGCTTA164150.013.17006444
CGGACAC101250.012.89071913
CATAGGG171700.012.69654637
GTCACTC213200.012.6014188
ATAGGGG175900.012.49564638
TAGCGTA13750.012.43491937
TGGCATA175150.012.43415234
CCGGACA106000.012.27061112
GGGAGGT246800.012.2533653
ACGGACA102550.012.15646518
CACCCGG105400.012.1257164
GGTCACT221650.012.0395377
GCATAGG181100.012.01309236
ACTCTCC233450.011.61547111
Produced by FastQC (version 0.11.5)