FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46778746
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT688790.14724422069800674No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG512520.10956257784250993No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC468320.10011384229923563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC156050.023.847954
CAGACCG164700.022.7867223
GACCGCG170050.021.9241585
AACGCTA141500.021.86368223
TTCGACG15600.021.6343426
ACGCTAC145450.021.36274324
ACCGCGT180100.020.713236
CGCTACC141550.020.18695825
GTCGCTA41400.020.10844227
AAAACGC161400.019.16797421
GAAAACG155350.018.78476320
TCAGACC205050.018.6213512
CGCTATG46650.017.79731829
TCGCTAT47550.017.69701428
AAACGCT178100.017.4464422
GTTTTCG186000.016.82692345
TTCGGCG133950.016.51206244
TCGGCGC136000.016.2797145
ATGAACG52650.016.11101233
TCGTGGT196450.015.87456140
Produced by FastQC (version 0.11.5)