FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46778746
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG846080.18086846534962694No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA587140.12551426667144946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG235450.017.55244345
GTCACTC341100.016.5187328
GGCATAG253400.015.9983735
GGTCACT352550.015.9643427
AGGTCAC368600.015.5263546
GAGCTTA265300.015.51818644
ACTCTCC371050.015.33087411
TGGCATA271500.014.97324434
ATAGGGG278300.014.74479538
TAGCGTA14900.014.64584437
GTTGTCG30450.014.481359526
ATAGCTC97750.014.38441639
ACCCGGC108850.014.371215
GGGAGGT431600.014.3219463
CATAGGG287650.014.24993637
GAGGTCA405050.014.2032975
GGAGGTC410700.014.0745114
TCACTCT411850.013.8832059
GCATAGG303000.013.46859236
CACTCTC420600.013.45528310
Produced by FastQC (version 0.11.5)