FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52570397
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG148150.014.98236645
GGCATAG155050.014.3815635
ATAGGGG165600.013.587438
ACCCGGC76650.012.4148965
CATAGGG180650.012.40560837
GTTGTCG22400.012.35588926
TGGCATA182850.012.34276834
GGGAGGT276200.012.3159693
GAGCTTA182300.012.29128944
GCATAGG187550.011.98526436
GGAGGTC284650.011.1121934
TCACCCG88600.011.0711633
ATAGCTC84850.010.34224539
TAGGGGA292700.010.21655139
CGGGACG48700.010.20900817
GTCAGGA215050.010.0704011
ACTCTCC311450.09.93909911
CGGACAC100750.09.71323913
ACGGACA99950.09.52093218
ACGGAAC63050.09.4553086
Produced by FastQC (version 0.11.5)