FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48955255
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1159750.2369000018486269No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1157940.23653027647389438No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1045280.2135174252488318No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG615460.1257188834988195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT479900.016.4287193
GTTGTCG42150.016.22801826
GGAGGTC496400.015.7377694
GGTCACT510900.014.9584297
GTCACTC514500.014.86393458
GAGGTCA544000.014.5084895
AGGTCAC541350.014.4083456
TGGGAGG409850.014.2303342
ACTCTCC558900.013.76732211
CACCCGG120300.013.5762411
AGCTTAG413950.013.52030645
TCACTCT575850.013.401659
CTGGGAG466650.013.386461
CACTCTC577700.013.16419110
ATATAGC59700.012.9220956
GGCATAG434250.012.88219435
ATAGGGG439650.012.84819238
GCTAAGT55650.012.73653414
ACCCGGG127950.012.5180692
TATAGCT64350.012.512187
Produced by FastQC (version 0.11.5)