FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57660081
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28300.015.42366826
ATAGGGG171350.012.69712338
AGCTTAG175050.012.32592345
CACCGAA296050.012.03036545
GGCATAG179000.011.89052535
GCACCGA303000.011.71729344
GGGAGGT279100.011.5762643
ATATAGC48800.011.526426
GTCACTC270700.011.1214868
GAGCTTA198000.010.91996244
GGTCACT271800.010.8526397
GGAGGTC289300.010.7714764
TTCTATA369050.010.3886012
ACTCTCC287900.010.32465711
TGGCATA211050.010.2447434
TCTATAG357050.010.2336563
CATAGGG211350.010.14503637
TAATCGG51850.010.0673024
CTATAGT366250.09.9950224
GCATAGG219650.09.77192436
Produced by FastQC (version 0.11.5)