FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51197329
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT88650.019.06421745
ACCGCGT91600.018.4212916
AGACCGC92050.018.2588374
TTCGACG20900.017.869656
GACCGCG94700.017.7711895
CAGACCG102300.016.7372973
CCGCGTT102000.016.5871897
AACGCTA54200.015.98165123
ACGTTCG23500.015.7011076
GTCCGTT40650.015.6707491
CGCGTTC108050.015.5960838
ACGCTAC56300.015.38553224
AGAACGA114600.014.90440144
TACGGAG114000.014.90124834
ATACGGA115100.014.719254533
AATACGG115350.014.60897532
TCGGCGC71600.014.48924245
TTCGGCG75900.014.14275244
TCGACGA25400.014.0837567
CCCGTAT33400.013.68086051
Produced by FastQC (version 0.11.5)