FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51197329
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31850.016.52883326
AGCTTAG122000.014.01955645
GGCATAG125450.013.68501535
ACCCGGC94200.013.1631675
ATAGGGG136850.012.742599538
CATAGGG135750.012.59701637
TGTCGTT43600.012.33229328
GAGCTTA144950.011.87747544
TCACCCG110100.011.8139273
GCATAGG146250.011.70794236
ATAGCTC104600.011.68123639
ACGGACA113200.011.3093318
ATATAGC55350.011.2600296
GTCACTC208150.011.2414748
CGGACAC113500.011.22007713
GGTCACT211950.011.082397
TGGCATA157250.010.96024334
CGGACAG115900.010.81209119
ACACGGA116750.010.7149216
GCTAAGT49800.010.6626814
Produced by FastQC (version 0.11.5)