FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51901331
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1628850.3138358821664901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT278100.028.91211145
AGACCGC281600.028.8189564
ACCGCGT290050.027.9704286
CAGACCG291700.027.9213543
GACCGCG291300.027.8515915
CCGCGTT301650.026.8873677
AGAACGA308300.026.17299344
AATACGG308550.026.02649332
ATACGGA311100.025.7752133
TACGGAG312750.025.58857234
CGCGTTC317900.025.5830388
TCAGACC335850.024.7470782
CAATACG328600.024.63010631
AAGAACG326850.024.58384543
ACGGAGA349150.022.98638235
GCGTTCT363000.022.478879
CCCAATA369450.022.0507929
CGGAGAG367400.021.80168236
CTCAGAC419000.021.7868961
CCAATAC399050.020.61817730
Produced by FastQC (version 0.11.5)