FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51901331
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG849170.1636123744109761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG241650.017.5554345
ATAGGGG255900.016.75968438
GGCATAG255650.016.6368935
GTCACTC363450.016.3218968
GGTCACT365350.016.0598937
GGGAGGT390000.016.0295853
GAGCTTA273550.015.56297544
ACTCTCC383100.015.37145511
TGGCATA285850.015.12406234
AGGTCAC404200.014.8232116
GGAGGTC412900.014.6338554
CATAGGG295500.014.43780437
GAGGTCA429450.014.1999745
GTTGTCG27050.013.47528126
GCATAGG318350.013.43700136
TGGGAGG355700.012.934882
TCACTCT464800.012.8839219
TAGGGGA450500.012.87447239
TCTCCCC470550.012.60751713
CACTCTC471250.012.49725710
Produced by FastQC (version 0.11.5)