FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49725236
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1244500.25027533303210464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG21250.023.2936616
TCACGCT313450.022.2949858
AACGCTA311700.021.8787823
ACGCTAC313300.021.78859324
CGCTACC325800.020.88356625
AAAACGC334000.020.50558521
TTCACGC347650.020.4770897
CCGGCTT351700.020.4593522
AGACCGC99950.020.440254
GAAAACG337850.020.16535820
GACCGCG103450.019.813955
ACCGCGT103300.019.7769586
ACGCTCA363500.019.67086610
CACGCTC360400.019.5716199
GCCGGCT343500.019.5257661
AAACGCT362150.018.9303222
CAGACCG111700.018.4512353
CGGCTTC419900.018.0686153
GTTTTCG389850.017.37181345
CCGCGTT120250.017.0079827
Produced by FastQC (version 0.11.5)