FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41892015
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCAC455880.10882264794376685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTAC115400.019.00656525
GTCCGTT37450.017.4386231
CGCATTA42350.016.83476320
GATTTAT143700.016.6927385
TAATACA134700.016.5604718
CGTATAT46100.016.3881363
AGCACTA137650.016.22724
CACTACA140700.015.76423326
CTCGGTT78600.015.7611351
CTCGTAT70900.015.7002451
CCCAGAT185350.015.6195351
TAACCCC46150.015.50206825
AGATTTA140400.015.3127124
AACGCTA76950.015.12502823
ATAATAC149300.015.00119817
CTCCGTT58150.014.8201471
ACGCTAC79250.014.68666524
GCGTACG13950.014.62866323
ACTACAG153750.014.61622327
ATGCGCG19800.014.60587944
Produced by FastQC (version 0.11.5)