FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41892015
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG48050.011.52087915
CGCGTTC49600.011.11545714
TAGCGTA6950.011.0056537
ATAGGGG72750.010.94691838
CCGCGTT51150.010.86657413
CGTTCGC53550.010.29557316
CATAGGG73750.010.21890537
GGCATAG73650.010.14120435
ATAGCTC64600.09.99472739
GCATAGG75500.09.89268836
ACCCGGC69550.09.8031135
AGCTTAG79750.09.73217745
CGTAACG14550.09.43166642
CTAGAAT66750.09.3374621
TTCGCAC60350.09.28461518
GAGCTTA87500.09.15300144
ACGGACA79800.08.74179618
TAATCGG39200.08.7250464
CGGACAG78100.08.70127119
CCGGACA82050.08.639194512
Produced by FastQC (version 0.11.5)