FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53445822
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT736450.13779374559904795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT179050.020.28378745
AGACCGC184400.019.6573434
ACCGCGT183000.019.5842866
GACCGCG184950.019.379585
TTCGACG22950.019.1159086
CGCGTTC195450.018.3943378
CAGACCG199950.018.3539033
CCGCGTT195700.018.336357
AGAACGA228700.016.0376744
AATACGG227950.015.87209332
ATACGGA228900.015.84568733
AACGCTA148350.015.78750623
TCAGACC239300.015.7495442
ACGCTAC150600.015.5217624
TACGGAG235250.015.3127634
CGCTACC156950.014.88083825
GCGTTCT246950.014.6402989
CAATACG253600.014.34728231
TCGACGA31100.014.3234577
AAGAACG265150.013.80752243
Produced by FastQC (version 0.11.5)