FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53445822
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG675610.12641025523005334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN622400.11645437879129261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG215050.016.27101145
GGCATAG234450.014.98317935
ATAGGGG246350.014.60620638
GTCACTC313450.014.1508118
GAGCTTA252250.013.9606644
GGTCACT321000.013.8456587
GGGAGGT367150.013.6351133
CATAGGG261200.013.43170637
AGGTCAC347850.013.1646016
TGGCATA270850.013.144434
GGAGGTC351150.013.1159624
ACTCTCC343600.012.8840911
GCATAGG273650.012.82891236
GAGGTCA373900.012.4276985
TCACTCT390000.011.5638019
CACTCTC379400.011.55534410
GTTGTCG30200.011.32611726
TAGGGGA400600.011.29626739
ACCCGGC87650.011.2392685
TGGGAGG355300.010.7922512
Produced by FastQC (version 0.11.5)