FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49318437
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24800.019.142726
AACGCTA84850.018.82682623
ACGCTAC87650.018.2510924
ACCGCGT92450.017.5225946
GACCGCG93500.017.349885
AGACCGC94200.017.1493154
CGCTACC93350.017.11266925
CGGTATA40900.016.66824720
CAGACCG101600.016.2988663
TTCGGCG79800.016.29672844
TCGGCGC82000.016.13388445
AAAACGC104000.015.48996121
CCGCGTT104450.015.4663087
TCGACGA30750.015.3654327
CGCGTTC107200.015.06955058
GAAAACG115850.014.00263820
GTCCGTT42000.013.6202341
CGTACGC97900.013.6054155
TCACGCT129800.013.381768
CCGTACG99350.013.2936284
Produced by FastQC (version 0.11.5)