FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53175958
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT46550.018.190761
TTCGACG22100.015.8819416
CCCGTAT37300.015.8188571
AACGCTA115250.015.30547423
GTCCGAT32250.015.2931531
ACGCTAC117950.015.06956824
CTCGGTT120550.015.0387351
CGCTACC121750.014.46986425
CTCCGGT73750.014.3827361
GTCGGTT46100.014.3624681
CCCGGAT112800.014.0754621
AGACGCT201350.013.88976241
CCCCGCA196850.013.521378535
ATGCGCG18150.013.51209644
CCCCCCT40950.013.4739481
AAGCGGC198000.013.31783628
CTCCGTT82700.013.3163751
CCTTACG52450.013.25526241
TCGACGA28700.013.1703897
TCGGTTT136550.013.1157342
Produced by FastQC (version 0.11.5)