FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53175958
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG127450.012.08994345
GGCATAG127000.011.93803835
ATAGGGG130400.011.93724338
CATAGGG135050.011.27637937
GCATAGG140450.010.84282636
GAGCTTA153950.010.33030744
TGGCATA164850.09.48365934
GGGAGGT212100.09.4008593
GTCACTC202400.09.3738528
TCAGACG80800.09.2190682
TAGCGTA8850.09.15028937
GGAGGTC214900.09.037584
TAATCGG36600.08.9773794
TAGGGGA206100.08.88424139
ACTCTCC218700.08.8399111
GGTCACT212100.08.5843717
ACCCGGC72750.08.5380025
ATCGGTT43050.08.20776756
GTAACGA37750.07.928211718
TCGCGCT29150.07.793926745
Produced by FastQC (version 0.11.5)