FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55895375
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1552980.27783694089180005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG144850.013.46607145
ATAGGGG156100.012.78868738
GGCATAG151300.012.75901435
CATAGGG163600.011.99232937
GAGCTTA168550.011.70953944
GTCACTC225500.011.4969638
GCATAGG173100.011.17738236
GGTCACT234100.011.0363497
AGGTCAC249850.010.8085786
TGGCATA182050.010.7882834
GGGAGGT275300.010.38948253
ACTCTCC252500.010.23875911
GTTGTCG31000.09.79619526
GGAGGTC280600.09.7294034
GAGGTCA291300.09.5724785
CGTCGAT25450.09.37156712
ATAGCTC83200.09.11622439
TAGGGGA281300.09.081017539
TCAGACG93000.09.0262412
ACCCGGC96300.08.7405525
Produced by FastQC (version 0.11.5)