FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50215876
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG23800.021.079846
AACGCTA164650.017.19110323
ACGCTAC165300.017.16450924
AGACCGC72000.016.8759274
ACGTTCG27350.016.7807836
CCCGTAT31900.016.6777921
ACCGCGT73600.016.6287846
TGCGCGT28750.016.58970645
TCGACGA30850.016.5542727
CGCTACC176700.016.04459425
TCACGCT186700.015.7978418
ACGGTAC136500.015.74024245
GACCGCG77500.015.7070965
ACGTCCG21050.015.49934232
AAAACGC183900.015.41523821
TACGGTA141750.015.09378344
ATGCGCG32250.014.78927344
CGACGAG31800.014.7862668
GTCCGTT36300.014.7183451
CCGCGTT83600.014.532057
Produced by FastQC (version 0.11.5)