FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50215876
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC97450.015.489825
AGCTTAG159000.014.5866245
GGCATAG165700.014.082540535
TCACCCG112750.013.610133
ACGGACA113800.013.16883118
CATAGGG183450.012.96528337
CGGACAC119700.012.89144113
CACCCGG119700.012.8000564
TCAGACG100800.012.7458872
GAGCTTA183200.012.69673744
GCATAGG192500.012.41419436
CCGGACA123250.012.33713612
GTCACTC237400.012.192798
ATAGGGG196550.012.16935838
CGGACAG121150.012.14701519
TGGCATA198500.012.0050334
GGTCACT247050.011.8818767
ACACGGA128350.011.63985616
CACGGAC130050.011.60987117
ACTCTCC251950.011.38949211
Produced by FastQC (version 0.11.5)