FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51385249
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1017480.1980101332193603No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG678820.13210405966895286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT252500.021.3966146
AACGCTA203900.021.07251423
AGACCGC260250.021.0705764
ACGCTAC203850.021.05569324
GACCGCG255950.021.0466925
CAGACCG269350.020.4756133
CGCTACC212700.020.17974925
CCGCGTT268300.020.1533497
CGCGTTC275250.019.603728
TTCGACG25850.019.4904086
AAAACGC225650.019.16916321
GAAAACG221150.018.63349720
TCAGACC317850.018.2499892
AAACGCT242550.017.87989622
GAACGAT316650.017.52340945
TTCACGC258650.017.3659697
TCACGCT257550.017.3354268
GCGTTCT315000.017.1441979
CCGGCTT277700.016.7983382
ACGCTCA271250.016.63410
Produced by FastQC (version 0.11.5)