FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51385249
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG172550.012.798202545
GTTGTCG41550.012.78184626
ATAGGGG198850.011.37014738
GGGAGGT298650.011.2980063
GTCACTC276150.011.0736648
GGCATAG200300.010.93247335
GGTCACT279750.010.8584837
AGGTCAC293100.010.7086546
GAGCTTA214400.010.28502244
GAGGTCA310600.010.2600935
ACTCTCC297750.010.166532511
TGGCATA229850.09.65426734
GGAGGTC353600.09.622984
TATAGCT66750.09.5664547
TCACTCT331250.09.4902379
GCTAAGT61250.09.25996314
CATAGGG243800.09.21933737
TGTCGTT58550.09.07108728
CACTCTC339900.08.79342410
TACAATA79550.08.7672892
Produced by FastQC (version 0.11.5)