FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50513011
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG638430.12638921880938755No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT510070.1009779440786058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG21850.022.9612396
ACGTCCG31200.021.99585532
ACCGCGT78350.020.8468046
AGACCGC82950.019.744994
GACCGCG82750.019.7111455
ACGCTAC190350.019.18495824
AACGCTA191050.019.13607823
TCGTATG37200.018.8707945
TCACGCT202250.018.6323728
CGCTACC199500.018.2712125
CCGCGTT89650.018.1187747
CAGACCG96100.017.277263
TCGACGA30400.017.2434527
ACGTTCG28500.017.1299766
ACGCTCA228450.016.98790610
AAAACGC226000.016.28608721
TGCGCGT36450.016.23441545
CACGCTC240400.015.9656469
GAAAACG232500.015.70498420
CGCGTTC103850.015.6629548
Produced by FastQC (version 0.11.5)