FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50513011
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG553760.10962720080178946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG181300.015.64311445
GGCATAG185550.015.50016535
TCACCCG119400.014.623583
ACGGACA119450.014.23927518
TAGCGTA13350.013.9916637
GAGCTTA205600.013.94686144
TGGCATA209200.013.84459634
GGTCACT295000.013.6760457
TCAGACG89450.013.5910792
GTCACTC301750.013.4826658
CGGACAC128350.013.25547613
ACTCTCC299350.013.25253611
CATAGGG218700.013.19203337
CGGACAG128400.013.07184319
ACCCGGC133350.012.9667115
GGAGGTC318250.012.9146394
GGGAGGT364200.012.8462923
ATAGGGG226450.012.76031138
AGGTCAC329150.012.6102776
CCGGACA135700.012.586802512
Produced by FastQC (version 0.11.5)