FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55234768
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1719460.3113003027368559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT276250.029.44253745
AGAACGA330600.024.62857244
AGACCGC339800.023.970764
ACCGCGT340800.023.9484796
GACCGCG344750.023.6984565
CCGCGTT347300.023.5069127
CAGACCG349050.023.4000573
CGCGTTC349100.023.3921958
AATACGG363450.022.41862132
ATACGGA370000.022.0825533
TACGGAG372550.021.81063734
TCAGACC394450.020.9814242
GCGTTCT401650.020.4268889
ACGGAGA402750.020.19193335
AACGCTA64450.019.82579223
TTCGGCG129300.019.6100644
CCCAATA419200.019.51226829
AAGAACG419600.019.40398243
CAATACG423600.019.2936631
ACGCTAC67050.019.15766124
Produced by FastQC (version 0.11.5)