FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55234768
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC589790.10677875934954593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC165450.018.6521155
ACGGACA179750.017.1727118
TCACCCG188100.016.932473
CGGACAC182050.016.84315913
CACCCGG193350.016.2053284
CGGACAG192350.016.08302719
ATAGCTC190050.015.66533739
TAGCGTA19300.015.51731637
CCGGACA200600.015.47487612
GACACGG205750.014.8820215
ACACGGA204800.014.87434616
CACGGAC209250.014.80526717
GCCCGGA214850.014.65466810
TAGCTCT205550.014.52803840
TGATAGC216950.014.16756737
AGCTTAG122100.013.93447845
TTGATAG223800.013.88101236
ATAGCGT22300.013.72891436
GGCATAG126000.013.56681235
GATTGAC227700.013.53603726
Produced by FastQC (version 0.11.5)