FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43960665
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG494560.11250057295539091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG152200.016.19660445
GGCATAG162950.015.05751935
GTTGTCG29150.014.29578726
CATAGGG179400.013.85240937
GAGCTTA180150.013.79565144
TAGCGTA13150.013.68200537
ATAGGGG182650.013.64286438
TGGCATA185700.013.382380534
GCATAGG192350.012.9548136
ATATAGC37800.012.9130016
ATAGCTC81950.012.76115439
ACCCGGC88900.012.7099525
GTCACTC271900.012.2870678
ACTCTCC276400.012.21841111
TGTCGTT34200.012.18484528
GGGAGGT308500.012.1401133
TATAGCT42450.012.029957
ACGGACA92300.011.99083218
GGAGGTC286850.011.8168484
GGTCACT279950.011.7726127
Produced by FastQC (version 0.11.5)