FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004852188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004852188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50586621
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG640350.12658485333503497No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT624780.1235069644204937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG17100.018.9467166
ACGCTAC193750.018.33614524
AACGCTA195450.018.1881723
CGCTACC197500.017.95381425
TCACGCT207650.017.8346868
CCGGCTT238850.016.6353932
ACGCTCA224550.016.62267510
TTCACGC224600.016.5488537
ACCGCGT37750.016.4497666
TCGGCGC92550.016.33655445
GCCGGCT222700.016.2481271
TTCGGCG94650.016.06917844
CCGTACG98400.015.8683574
CCCGTAT33650.015.8017411
CGTACGC100400.015.7091235
GTTAGTT196150.015.53094844
CTCGCGT10900.015.2961891
AAAACGC235800.015.19031721
AGACCGC41550.015.1619354
CACGCTC247650.015.081259
Produced by FastQC (version 0.11.5)